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UIC
Chemistry NMR / Undergraduate Teaching Labs
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Click here to read our page on Sample Preparation Tips (html page). This is relevant for all lab classes using NMR. Jump to: Chem-130 | Chem-222 | Chem-235 | Chem-343 | Chem-415 | Chem-421 | Chem-452 Chem-235 Probably the largest area of application of NMR spectroscopy is in support of organic chemistry. NMR can be used both as a method of checking e.g. the progress of a reaction and the purity of its products, and as a tool for structure determination of organic molecules. To this end, both proton (H-1) and carbon-13 (C-13) NMR are of great importance, the latter particularly in the area of structure determination. In Chem-235 we provide students with an introduction to the use of both H-1 and C-13 NMR spectroscopy in organic chemistry. To do this we will obtain H-1 and C-13 spectra of student samples obtained from two different synthetic projects. In the lab, students may choose to carry out the synthesis of either lidocaine or an octanol isomer. After completing the synthesis, each group will make take a sample of their final product, dissolve it in CDCl3, and filter the resulting solution into an 8"-long 5mm NMR tube.These tubes will then be submitted to the NMR lab. After the samples are run, each group will get H-1 and C-13 NMR spectra (hardcopy) of their own sample - so you can see how good of a job you did with your synthesis! As an example, below we show some spectra obtained from student samples during an overnight run on our Varian Mercury-Vx 300. These are unretouched actual spectra direct from the spectrometer! Lidocaine in CDCl3:   Octanol isomer (you decide which) in CDCl3:   Questions or comments, email Dan McElheny or Prof. Krumpolc (Director of Organic Laboratories). Back to top. Chem-343 In this class you will learn how to use the Varian Mercury-Vx-300 spectrometer for 1D H-1 and C-13 spectra. After a lab meeting for instruction and practice, you will then carry out a series of NMR experiments designed to exemplify the phenomenon of polarization transfer, which is an important part of modern NMR. Along with this, you will also learn how to obtain a 2D H-1/C-13 heteronuclear correlation spectrum, and you will use this spectrum to help make chemical shift assignments of a small organic molecule. Click here to get the instruction book which covers basic instrument operation, 2D experiments, and the polarization-transfer lab (PDF format): Instructions for the NMR Laboratory - Chem-343. Here is a page showing the structures of the training samples we will use: NMR sample structures - Chem-343. Back to top. Chem-421 In this laboratory you will gain more practice in the routine operation of four types of experiments for modern pulsed nuclear magnetic resonance (NMR) spectroscopy. The operation of the instrument is covered in materials that are linked below. Please download, print out, and review these materials before coming to lab. You will have one or more unknowns to identify, draw their structures and annotate the various spectra. Each is a common pharmaceutical. The experiments that are available to you are H-1, C-13, C-13 DEPT, H1- COSY and H1-C13-HETCOR. To interpret their spectra, please refer to Chapter 11 of your text (or your lecture notes) for a more detailed description. Questions for lab report will be handed out during the laboratory. Click here to get the instruction book (PDF format): Basic 1D and 2D experiments on the Varian Mercury-Vx-300. Back to top. We would very much like to acknowledge funding for the purchase of the Varian Mercury-Vx console and sample-changer, which was obtained from:
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